TGF-beta RII Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TGF-beta RII Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII
  Ile24-Asp159
  Accession # P37173
• Specificity: Detects human TGF-beta RII in ELISAs and Western blots. In sandwich immunoassays, approximately 25% cross-reactivity with recombinant human (rh) TGF-beta RIIb is observed and less than 0.05% cross-reactivity with rhTGF-beta RI, rhTGF-beta RIII, recombinant mouse TGF-beta RII, and rhTGF-beta 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGFBR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human TGF-beta RII Antibody (Catalog # AF-241-NA)
  ELISA Detection: Human TGF-beta RII Biotinylated Antibody (Catalog # BAF241)
  Standard: Recombinant Human TGF-beta RII (Catalog # 241-R2)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .

1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFBR2