Human TGF-alpha Recombinant Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
LYOPH

Order Details

Human TGF-alpha Recombinant Protein Summary

Description
A single, non-glycosylated polypeptide corresponding to amino acids Val40 - Ala89 of TGFA.

Source: E. coli

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
The ED50, as determined by a cell proliferation assay using murine Balb/3T3 cells, is less than 0.4 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TGFA
Purity
SDS-PAGE
Endotoxin Note
Less than 1 EU/mg of the rHuTGF-a as determined by LAL method.

Applications/Dilutions

Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration
LYOPH
Purity
SDS-PAGE
Reconstitution Instructions
Reconstitute with 10 mM acetic acid to a final concentration of 0.1 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human TGF-alpha Recombinant Protein

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

TGF-a is an EGF family cytokine that is expressed as a transmembrane protein and as a shed soluble molecule. It is expressed in pituitary, brain, keratinocytes, macrophages, and many tumors. TGF-a is functional across species and triggers the activation of the EGF receptor. It promotes fibroblast proliferation, keratinocyte migration, epithelial development, and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TGFA