Human TGF-alpha Biologically Active Protein Summary
A single, non-glycosylated polypeptide corresponding to amino acids Val40 - Ala89 of TGFA.
Source: E. coli
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
The ED50, as determined by a cell proliferation assay using murine Balb/3T3 cells, is less than 0.4 ng/ml.
Less than 1 EU/mg of the rHuTGF-a as determined by LAL method.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Reconstitute with 10 mM acetic acid to a final concentration of 0.1 mg/ml.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Human TGF-alpha Biologically Active Protein
- protransforming growth factor alpha
- transforming growth factor, alpha
- transforming growth factor-alpha
TGF-a is an EGF family cytokine that is expressed as a transmembrane protein and as a shed soluble molecule. It is expressed in pituitary, brain, keratinocytes, macrophages, and many tumors. TGF-a is functional across species and triggers the activation of the EGF receptor. It promotes fibroblast proliferation, keratinocyte migration, epithelial development, and angiogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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