Mouse TGF-alpha ELISA Kit (Chemiluminescence)

Images

 
Standard Curve Reference.
Samples were spiked with high concentrations of Mouse TGF-alpha, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL

Order Details

Mouse TGF-alpha ELISA Kit (Chemiluminescence) Summary

Description
Assay Length: 3 h 30 min
Specificity
This kit recognizes Mouse TGF-alpha in samples. No significant cross-reactivity or interference between Mouse TGF-alpha and analogues was observed
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
TGFA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate(100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro CLIA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Substrate Reagent A
  13. Substrate Reagent B

Alternate Names for Mouse TGF-alpha ELISA Kit (Chemiluminescence)

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for TGF-alpha ELISA Kit (NBP3-49958). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol TGFA