Human TGF-alpha ELISA Kit (Colorimetric)

Images

 
Human TGF-alpha ELISA Kit (Colorimetric) [NBP2-60146]
Human TGF-alpha ELISA Kit (Colorimetric) [NBP2-60146] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
62.5 pg/mL - 4000 pg/mL
Sensitivity
15.6 pg/mL

Order Details

Human TGF-alpha ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Standard Curve Range
62.5 pg/mL - 4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TGFA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 N HCI
  2. 1.2 N NaOH/0.5 M HEPES
  3. Adhesive Strip (For 96 wells)
  4. Assay plate (12 x 8 coated Microwells)
  5. Biotin-antibody (100 x concentrate)
  6. Biotin-antibody Diluent
  7. HRP-avidin (100 x concentrate)
  8. HRP-avidin Diluent
  9. Instruction manual
  10. Sample Diluent
  11. Standard (Freeze dried)
  12. Stop Solution
  13. TMB Substrate
  14. Wash Buffer (25 x concentrate)

Alternate Names for Human TGF-alpha ELISA Kit (Colorimetric)

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

236-EG
Species: Hu
Applications: BA
AF231
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
M6000B
Species: Mu
Applications: ELISA
233-FB
Species: Hu
Applications: BA
294-HG
Species: Hu
Applications: BA
291-G1
Species: Hu
Applications: BA
AF262
Species: Hu
Applications: ICC, IHC, Neut, WB
DVE00
Species: Hu
Applications: ELISA
7754-BH/CF
Species: Hu
Applications: BA
251-KG
Species: Hu
Applications: BA
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
1129-ER
Species: Hu
Applications: BA
NB500-106
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow

Publications for TGF-alpha ELISA Kit (NBP2-60146) (0)

There are no publications for TGF-alpha ELISA Kit (NBP2-60146).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TGF-alpha ELISA Kit (NBP2-60146) (0)

There are no reviews for TGF-alpha ELISA Kit (NBP2-60146). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TGF-alpha ELISA Kit (NBP2-60146) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TGF-alpha Products

Research Areas for TGF-alpha ELISA Kit (NBP2-60146)

Find related products by research area.

Blogs on TGF-alpha

There are no specific blogs for TGF-alpha, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Human TGF-alpha ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol TGFA