Human TGF-alpha ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Standard Curve Range |
62.5 pg/mL - 4000 pg/mL |
Sensitivity |
15.6 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
TGFA |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 1 N HCI
- 1.2 N NaOH/0.5 M HEPES
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Human TGF-alpha ELISA Kit (Colorimetric)
Background
Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: ICC, IHC, Neut, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
Species: Hu, Mu
Applications: ICC/IF, WB
Species: Hu
Applications: BA
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Publications for TGF-alpha ELISA Kit (NBP2-60146) (0)
There are no publications for TGF-alpha ELISA Kit (NBP2-60146).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for TGF-alpha ELISA Kit (NBP2-60146) (0)
There are no reviews for TGF-alpha ELISA Kit (NBP2-60146).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for TGF-alpha ELISA Kit (NBP2-60146) (0)
Additional TGF-alpha Products
Research Areas for TGF-alpha ELISA Kit (NBP2-60146)
Find related products by research area.
|
Blogs on TGF-alpha