TGF-alpha Antibody (JB58-36)

Immunocytochemistry/Immunofluorescence: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF-alpha antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF-alpha antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGF-alpha Antibody (JB58-36) [NBP2-75682] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-alpha antibody. Counter stained with hematoxylin.

Product Discontinued

TGF-alpha Antibody (JB58-36) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human TGF-alpha aa 1-160 / 160. (SwissProt: P01135 Human)
• Localization: Plasma membrane. Extracellular region. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TGFA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:50
  • Western Blot 1:500
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TGF-alpha Antibody (JB58-36)

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFA