TGF-alpha Antibody (1E8-269)

Product Discontinued

TGF-alpha Antibody (1E8-269) Summary

• Immunogen: 32-40 AA sequence linked to thyroglobulin.
• Localization: Cell Membrane (precursor) and Secreted (mature)
• Specificity: TGF alpha (1E8-269)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFA
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
• Application Notes: ELISA: Use at a dilution of 1/10. Is unsuitable for IHC-P, IHC-Fr or IHC-R.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  TGF-alpha Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and weakly with Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant with 0.2M Tris/HCl (pH 7.4) and 5 - 10 fetal Bovine serum (FBS)
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for TGF-alpha Antibody (1E8-269)

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFA
• Entrez:
  • 7039(Human)
  • 21802(Mouse)
• Uniprot:
  • P01135()