TET3 Antibody - BSA Free

Western Blot: TET3 Antibody [NBP3-18691] - Figure 3. Western blot analysis using the antibody directed against TET3 Whole cell extracts (25 ug) from Jurkat cells were analysed by Western blot using the antibody against TET3 diluted 1:200 in TBS- Tween containing 5% skimmed milk. Lane 2 shows the results after incubation of the antibody with the immunizing peptides. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Western Blot: TET3 Antibody [NBP3-18691] - Figure 2. Western blot analysis using the antibody directed against TET3 Whole cell extracts (25 ug) from Jurkat cells were analysed by Western blot using the antibody against TET3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

ELISA: TET3 Antibody [NBP3-18691] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against mouse TET3 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:20,300.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TET3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TET3 Antibody - BSA Free (NBP3-18691) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against TET3 (Tet Methylcytosine Dioxygenase 3), using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TET3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for TET3 Antibody - BSA Free

• EC 1.14.11.n2
• hCG_40738
• KIAA0401
• methylcytosine dioxygenase TET3
• MGC22014
• probable methylcytosine dioxygenase TET3
• tet oncogene family member 3

Background

TET3 may catalyze the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TET3
• Uniprot:
  • O43151(Human)