Recombinant Human TDP-43/TARDBP His Protein

SDS-Page: Recombinant Human TDP-43/TARDBP Protein [NBP2-22850]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human TDP-43/TARDBP His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-414 of Human TDP-43/TARDBP
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TARDBP
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 48.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TDP-43/TARDBP His Protein

• ALS10
• ALS10TAR DNA-binding protein-43
• TAR DNA binding protein
• TARDBP
• TDP43
• TDP-43
• TDP-43TAR DNA-binding protein 43

Background

TARDBP, as known as TAR DNA binding protein, was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It has also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. It has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recombinant human TARDBP protein protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for TDP-43/TARDBP Recombinant Protein (NBP2-22850). (Showing 1 - 2 of 2 FAQ).

1. Can I do the function test using this protein?
   • Unfortunately, we have not yet tested NBP2-22850 for functionality; therefore, we are unable to guarantee its performance in a functional assay.
2. Do you remove endotoxin from your recombinant human TDP-43 (Catalog # NBP2-22850)?
   • We do not prepare this protein for removal of the endotoxin other than the purification that is done standardly.   This protein is made only as a control for PAGE/WESTERN where the endotoxin would not matter rather than as an active protein.   We have not measured activity at all and would not be able to say if the protein has any activity.

Bioinformatics

• Gene Symbol: TARDBP