TDP-43/TARDBP Antibody

Images

 
Western Blot: TDP-43/TARDBP Antibody [NBP1-06995] - Staining of Human cerebellum (A), frontal cotex (B) and hippocampus (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more
Western Blot: TDP-43/TARDBP Antibody [NBP1-06995] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TDP-43/TARDBP Antibody Summary

Immunogen
Peptide with sequence C-HISNAEPKHNSNRQ corresponding to internal region according to NP_031401.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TARDBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB:Approx. 45kDa band observed in lysates of Jurkat (calculated MW of 44.7kDa according to NP_031401.1). A slightly larger protein is detected in some regions of the Brain. Recommended concentration: 0.3-1ug/ml..
Positive Control
TDP-43/TARDBP Lysate (NBL1-16701)
Publications
Read Publication using NBP1-06995.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TDP-43/TARDBP Antibody

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TARDBP
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TDP-43/TARDBP Antibody (NBP1-06995)(1)


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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TARDBP
Entrez
Uniprot