TCL1A Antibody (PD00-94)

Western Blot: TCL1A Antibody (PD00-94) [NBP3-33001] - Western blot analysis of TCL1A on different lysates with Rabbit anti-TCL1A antibody (NBP3-33001) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33001) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TCL1A Antibody (PD00-94) [NBP3-33001] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TCL1A antibody (NBP3-33001) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33001) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: TCL1A Antibody (PD00-94) [NBP3-33001] - Immunocytochemistry analysis of PC-3 cells labeling TCL1A with Rabbit anti-TCL1A antibody (NBP3-33001) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TCL1A antibody (NBP3-33001) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: TCL1A Antibody (PD00-94) [NBP3-33001] - Flow cytometric analysis of Daudi cells labeling TCL1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33001, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PD00-94
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TCL1A Antibody (PD00-94) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human TCL1A aa 1-114. (Uniprot: P56279)
• Localization: Cytoplasm, Nucleus, Microsome, Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TCL1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:1000
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TCL1A Antibody (PD00-94)

• Oncogene TCL1
• Oncogene TCL-1
• Protein p14 TCL1
• T-cell leukemia/lymphoma 1A
• T-cell lymphoma-1
• T-cell lymphoma-1A
• TCL1A
• TCL1-PEN
• TCL1T-cell leukemia/lymphoma protein 1A

Background

Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCL1A