TCF21 Antibody

Western Blot: TCF21 Antibody [NBP1-88637] - Analysis in control (vector only transfected HEK293T lysate) and tCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Product Discontinued

TCF21 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEESSNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQV
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCF21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation ChIP reported in scientific literature (PMID: 26020271).
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ChIP reported in scientific literature (PMID: 26020271). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26020271).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TCF21 Antibody

• bHLHa23BHLHA23
• Capsulin
• Class A basic helix-loop-helix protein 23
• epicardin
• Pod-1
• POD1pod-1
• Podocyte-expressed 1
• TCF-21
• transcription factor 21

Background

The basic helix-loop-helix transcription factor TCF21/Pod1 (also called capsulin or epicardin) is involved in kidney, lung and spleen organogenesis. It is also essential for normal development of the testes and ovaries. TCF21/Pod1 is involved in the transcriptional repression of steroidogenic factor 1 (Sf1/Nr5a1/Ad4BP), an orphan nuclear receptor that regulates the expression of multiple genes (including Scc) that mediate sexual differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF21 Antibody (NBP1-88637)(4)

Publications using NBP1-88637

• Coppiello G, Barlabé P, Moya-Jódar M et al. Generation of heart and vascular system in rodents by blastocyst complementation Developmental cell 2023-11-13 [PMID: 37967560] (IHC, Mouse)
  
  Details:
  Dilution 1:200
• He X, Smith SE, Chen S et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche Cell reports 2021-09-07 [PMID: 34496236]
• Nurnberg ST, Cheng K, Raiesdana A et al. Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap. PLoS Genet 2015-05-01 [PMID: 26020946] (IF/IHC, Human)
• Sazonova O, Zhao Y, Nurnberg S et al. Characterization of TCF21 Downstream Target Regions Identifies a Transcriptional Network Linking Multiple Independent Coronary Artery Disease Loci. PLoS Genet 2015-05-01 [PMID: 26020271] (Chemotaxis, Human)

Bioinformatics

• Gene Symbol: TCF21