TBX18 Antibody (635305) [Unconjugated]

TBX18 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TBX18 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and MCF‑7 human breast cancer cell line using Mouse Anti-Human TBX18 Monoclonal Antibody (Catalog # MAB63371) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 635305
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TBX18 Antibody (635305) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TBX18
  Ala384-Ser504
  Accession # O95935
• Specificity: Detects human TBX18 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX2, 3, 5, 6, 20, or rhT-bet is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBX18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBX18 Antibody (635305) [Unconjugated]

• T-box 18
• T-box protein 18
• T-box transcription factor TBX18
• TBX18

Background

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBX18 Antibody (MAB63371)(4)

Publications using MAB63371

• AC Silva, OB Matthys, DA Joy, MA Kauss, V Natarajan, MH Lai, D Turaga, AP Blair, M Alexanian, BG Bruneau, TC McDevitt Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids Cell Stem Cell, 2021-12-02;28(12):2137-2152.e6. 2021-12-02 [PMID: 34861147] (IHC, Human)
• Luoman Chen, Stephen Dalton Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells STAR Protocols 2020-10-28 [PMID: 33111082]
• T Colunga, M Hayworth, S Kre beta , DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 2019-03-05;26(10):2566-2579.e10. 3/5/2019 [PMID: 30840882]
• Caitlin M. Braitsch, Onur Kanisicak, Jop H. van Berlo, Jeffery D. Molkentin, Katherine E. Yutzey Differential expression of embryonic epicardial progenitor markers and localization of cardiac fibrosis in adult ischemic injury and hypertensive heart disease Journal of Molecular and Cellular Cardiology 2013-12-01 [PMID: 24140724]

Bioinformatics

• Gene Symbol: TBX18
• Uniprot:
  • O95935(Human)