TCF-3/E2A Antibody (6B8)

Western Blot: TCF-3/E2A Antibody (6B8) [NBP1-42566] - Analysis using TCF3 mouse mAb against A549 (1), A431 (2), Hela (3), PANC-1 (4) and PC-3 (5) cell lysate.

Flow Cytometry: TCF-3/E2A Antibody (6B8) [NBP1-42566] - Flow cytometry: analysis of A549 cells using anti-TCF3 mAb (green) and negative control (purple).

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 6B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TCF-3/E2A Antibody (6B8) Summary

• Description: Novus Biologicals Mouse TCF-3/E2A Antibody (6B8) (NBP1-42566) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human TCF-3/E2A expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Theoretical MW: 67.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for TCF-3/E2A Antibody (6B8)

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

T-cell factor 3 and 4 (TCF3 and TCF4) are transcription factors of the High Mobility Group of DNA binding proteins. They are members of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. TCF4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. TCF3 appears to be expressed independently of TCF4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF3