TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

Analysis in human kidney and tonsil tissues using NBP3-43951 antibody. Corresponding TCF-2/HNF-1 beta RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: TCF-2/HNF-1 beta Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Staining in A549 cell line with Anti-TCF-2/HNF-1 beta monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).

Staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.

Staining of human kidney shows strong nuclear positivity in cells in tubules.

Staining of human renal cancer shows strong nuclear positivity in tumor cells.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

Analysis in human cell lines Caco-2 and A-431 using Anti-TCF-2/HNF-1 beta antibody. Corresponding TCF-2/HNF-1 beta RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0374
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P35680, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

• FJHN
• hepatocyte nuclear factor 1-beta
• HNF1 beta A
• HNF-1 beta
• HNF1 homeobox B
• HNF1B
• HNF-1B
• HNF1beta
• HNF-1-beta
• HNF2
• Homeoprotein LFB3
• LFB3
• LF-B3
• MODY5
• MODY5HPC11
• TCF2
• TCF-2
• TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor
• Transcription factor 2
• transcription factor 2, hepatic
• Variant hepatic nuclear factor 1
• VHNF1

Background

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF1B