TCF-2/HNF-1 beta Antibody (CL0374) - BSA Free

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining in human kidney and tonsil tissues. Corresponding HNF1B RNA-seq data are presented for the same tissues.

Western Blot: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Analysis in human cell lines Caco-2 and A-431 using Anti-HNF1B antibody. Corresponding HNF1B RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.

Immunocytochemistry/Immunofluorescence: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining in A549 cell line showing spotty nuclear (without nucleoli)Staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.

Western Blot: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining of human kidney shows strong nuclear positivity in cells in tubules.

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining of human renal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody (CL0374) [NBP2-30678] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL0374
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TCF-2/HNF-1 beta Antibody (CL0374) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P35680, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TCF-2/HNF-1 beta Recombinant Protein Antigen (NBP2-30678PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for TCF-2/HNF-1 beta Antibody (CL0374) - BSA Free

• FJHN
• hepatocyte nuclear factor 1-beta
• HNF1 beta A
• HNF-1 beta
• HNF1 homeobox B
• HNF1B
• HNF-1B
• HNF1beta
• HNF-1-beta
• HNF2
• Homeoprotein LFB3
• LFB3
• LF-B3
• MODY5
• MODY5HPC11
• TCF2
• TCF-2
• TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor
• Transcription factor 2
• transcription factor 2, hepatic
• Variant hepatic nuclear factor 1
• VHNF1

Background

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF1B
• Uniprot:
  • P35680()