TCF-12/HTF4 Antibody

Images

 
Western Blot: TCF-12/HTF4 Antibody [NB100-2337] - Detection of Human TCF12 on HeLa whole cell lysate using NB100-2337. TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody NB100-2338.
Immunohistochemistry: TCF-12/HTF4 Antibody [NB100-2337] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TCF12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NB100-2337] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-TCF12 used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

TCF-12/HTF4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Transcription Factor 12 using the numbering given in entry NP_996919.1 (GeneID 6938).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TCF12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
TCF-12/HTF4 Lysate (NBL1-16771)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TCF-12/HTF4 Antibody

  • BHLHB20
  • bHLHb20E-box-binding protein
  • Class B basic helix-loop-helix protein 20
  • HEB
  • HEBDNA-binding protein HTF4
  • helix-loop-helix transcription factor 4
  • HsT17266
  • HTF4
  • HTF4TCF-12
  • TCF12
  • TCF-12
  • transcription factor 12
  • Transcription factor HTF-4

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TCF12
Entrez
Uniprot