TCERG1 Antibody - BSA Free

Western Blot: TCERG1 Antibody [NB300-214] - Detection of Human CA150 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CA150 antibody NB300-214 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: TCERG1 Antibody [NB300-214] - Section of human breast carcinoma (left) and mouse squamous cellcarcinoma (right). Antibody: Affinity purified rabbit anti-CA150 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: TCERG1 Antibody [NB300-214] - Nuclear extract (5 ug) from HeLa cells. Antibody used at 0.33 ug/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TCERG1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TCERG1 Antibody - BSA Free (NB300-214) is a polyclonal antibody validated for use in IHC and WB. Anti-TCERG1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a portion of human transcription elongation regulator 1 (CA150) encoded within exon 1 (LocusLink ID 10915).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCERG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Western Blot 1:1000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCERG1 Antibody - BSA Free

• CA150TATA box-binding protein-associated factor 2S
• co-activator of 150 kDa
• TAF2SMGC133200
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD
• TATA box-binding protein-associated factor 2S
• transcription elongation regulator 1
• Transcription factor CA150
• Urn1

Background

CA150 is a nuclear factor that represses RNA polymerase II (RNAPII) transcription by inhibiting the elongation of transcripts. It has also been shown to be involved in the age onset of Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCERG1 Antibody (NB300-214)(4)

Publications using NB300-214

• Jablonski JA, Amelio AL, Giacca M et al. The transcriptional transactivator Tat selectively regulates viral splicing. Nucleic Acids Res 2010-03-01 [PMID: 19966273]
• Kim D, Lee J, Cheng D et al. Enzymatic activity is required for the in vivo functions of CARM1. J Biol Chem 2010-01-01 [PMID: 19897492]
• Cheng D, Cote J, Shaaban S et al. The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing. Mol Cell 2007-01-01 [PMID: 17218272]
• McFie PJ, Wang GL, Timchenko NA et al. Identification of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha. J Biol Chem 2006-06-01 [PMID: 16644732]

Bioinformatics

• Gene Symbol: TCERG1
• Entrez:
  • 10915(Human)