GHITM Antibody

Images

 
Western Blot: GHITM Antibody [NBP1-47217] - Analysis of Human Cerebellum (A), Duodenum (B) and Heart (C) cell lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: GHITM Antibody [NBP1-47217] - Analysis of HeLa cell lysate (35ug protein in RIPA buffer) using NBP1-47217 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

GHITM Antibody Summary

Immunogen
Peptide with sequence C-PSREYATKTRIGIR corresponding to internal region according to NP_055209.2.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GHITM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 35 kDa band observed in lysates of HeLa and of human brain (cerebellum), duodenum and heart (calculated MW of 37.2 kDa band according to human NP_055209.2).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47217 in the following applications:

Publications
Read Publication using NBP1-47217.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GHITM Antibody

  • Dermal papilla-derived protein 2
  • DERP2MICS1
  • DKFZp566C0746
  • FLJ26584
  • growth hormone inducible transmembrane protein
  • growth hormone-inducible transmembrane protein
  • HSPC282
  • Mitochondrial morphology and cristae structure 1
  • PTD010
  • TMBIM5My021
  • transmembrane BAX inhibitor motif containing 5
  • Transmembrane BAX inhibitor motif-containing protein 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GHITM Antibody (NBP1-47217)(1)


Review for GHITM Antibody (NBP1-47217) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-47217:
Filter by Applications
WB
(1)
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Images Ratings Applications Species Date Details
Western Blot GHITM NBP1-47217
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reviewed by:
Anonymous
WB Mouse 06/22/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver mitochondria, Hela cell lysate
SpeciesMouse
LotP1

Blocking

Blocking Details5% milk TBS-T, 1 hour, Room temperature

Primary Anitbody

Dilution Ratio1:1000, 4°C overnight, 5% milk TBS-T

Secondary Antibody

Secondary Descriptionrabbit anti-goat IgG conjugated to HRP, Jackson ImmunoResearch in 2% milk TBS-T
Secondary Manufacturer Cat#305-035-003
Secondary Concentration1:10000

Details

Detection NotesSuperSignal West Pico ECL, exposure- 11.9 sec, BioRad ChemiDOC MP, positive control- Hela cell lysate

Comments

CommentsHela lysate shows expected band at ~37 kDa, mouse mitochondria band present at slightly lower size of ~35 kDa. Unspecific band detected at 55 kDa in mouse samples.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GHITM Antibody (NBP1-47217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GHITM Antibody (NBP1-47217)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
06/22/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GHITM
Entrez
Uniprot