TCEB1 Antibody (HL1683) - Azide and BSA Free

Western Blot: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - TCEB1 antibody [HL1683] detects TCEB1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. TCEB1 stained by TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TCEB1 Antibody (HL1683) - Azide and BSA Free [NBP3-25712] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (NBP3-25712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Ca, Dr, Fe
• Applications: WB, IHC
• Clone: HL1683
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TCEB1 Antibody (HL1683) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TCEB1 Antibody (HL1683) - Azide and BSA Free (NBP3-25712) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TCEB1. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ELOC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:5000-1:50000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TCEB1 Antibody (HL1683) - Azide and BSA Free

• ELOC
• elongin 15 kDa subunit
• elongin-C
• RNA polymerase II transcription factor SIII subuni
• RNA polymerase II transcription factor SIII subunit C
• SIII p15
• SIII
• TCEB1
• transcription elongation factor B (SIII), polypept
• transcription elongation factor B (SIII), polypeptide 1 (15kD, elongin C)
• transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• transcription elongation factor B polypeptide 1
• transcription elongation factor B subunit 1
• transcription elongation factor B, polypeptide 1

Background

Elongin C (also known as RNA polymerase II transcription factor SIII p15 subunit and transcription elongation factor B polypeptide 1) is a 15 kD member of the SKP1 family. Elongin functions as a regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. The Poly6131 antibody recognizes the human and mouse elongin C protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELOC