TCEB2 Antibody (6F6) - Azide and BSA Free

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01] - TCEB2 monoclonal antibody (M01), clone 6F6. Analysis of TCEB2 expression in PC-12.

Immunocytochemistry/Immunofluorescence: TCEB2 Antibody (6F6) [H00006923-M01] - Analysis of monoclonal antibody to TCEB2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: TCEB2 Antibody (6F6) [H00006923-M01] - TCEB2 monoclonal antibody (M01), clone 6F6 Analysis of TCEB2 expression in Hela S3 NE.

Sandwich ELISA: TCEB2 Antibody (6F6) [H00006923-M01] - Detection limit for recombinant GST tagged TCEB2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 6F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TCEB2 Antibody (6F6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TCEB2 (NP_009039, 9 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ
• Localization: Nuclear
• Specificity: TCEB2 - transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELOB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TCEB2 Antibody (6F6) - Azide and BSA Free

• 18-kD subunit
• EloB
• Elongin 18 kDa subunit
• elongin-B
• RNA polymerase II transcription factor SIII p18 subunit
• RNA polymerase II transcription factor SIII subunit B
• SIII p18
• transcription elongation factor B (SIII), polypeptide 2 (18kD, elongin B)
• transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
• transcription elongation factor B polypeptide 2

Background

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TCEB2 Antibody (H00006923-M01) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 08/27/2021

Summary

• Application: Western Blot
• Sample Tested: Colon tissue
• Species: Rat
• Lot: 6F6

Bioinformatics

• Gene Symbol: ELOB
• Entrez:
  • 6923(Human)
• OMIM:
  • 600787(Human)
• Uniprot:
  • NP_009039(Human)