TCEB1 Antibody (1076415) [Unconjugated]

TCEB1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

TCEB1 was detected in fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 1076415
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TCEB1 Antibody (1076415) [Unconjugated] Summary

• Immunogen: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex
  Accession # Q15369
• Specificity: Detects hElongin B/Elongin C/VHL/CUL2/RBX1 Complex in Direct ELISA
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCEB1 Antibody (1076415) [Unconjugated]

• ELOC
• elongin 15 kDa subunit
• elongin-C
• RNA polymerase II transcription factor SIII subuni
• RNA polymerase II transcription factor SIII subunit C
• SIII p15
• SIII
• TCEB1
• transcription elongation factor B (SIII), polypept
• transcription elongation factor B (SIII), polypeptide 1 (15kD, elongin C)
• transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• transcription elongation factor B polypeptide 1
• transcription elongation factor B subunit 1
• transcription elongation factor B, polypeptide 1

Background

Elongin C is a 12kDa, 112aa general transcription elongation factor.  Elongin C, along with Elongin B, Cul2 and Rbx1 to forms a complex with VHL, and Elongin C is required for the function of VHL.  Mutations in Elongin C are responsible for 0.5% to 5% of clear cell renal cell carcinomas.  Tumorigenic mutations frequently occur in a 35-residume domain of VHL responsible for Elongin C binding.

1. Brugarolas J. Molecular genetics of clear-cell renal cell carcinoma. J Clin Oncol. 2014 Jun 20;32(18):1968-76. doi: 10.1200/JCO.2012.45.2003. Epub 2014 May 12. PMID: 24821879; PMCID: PMC4050206.   
2. Stebbins CE, Kaelin WG Jr, Pavletich NP. Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function. Science. 1999 Apr 16;284(5413):455-61. doi: 10.1126/science.284.5413.455. PMID: 10205047.   
3. 3.Hakimi AA, Tickoo SK, Jacobsen A, Sarungbam J, Sfakianos JP, Sato Y, Morikawa T, Kume H, Fukayama M, Homma Y, Chen YB, Sankin A, Mano R, Coleman JA, Russo P, Ogawa S, Sander C, Hsieh JJ, Reuter VE. TCEB1-mutated renal cell carcinoma: a distinct genomic and morphological subtype. Mod Pathol. 2015 Jun;28(6):845-853. doi: 10.1038/modpathol.2015.6. Epub 2015 Feb 13. PMID: 25676555; PMCID: PMC4449825.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q15369(Human)