TBC1D4 [p Ser588] Antibody - Azide and BSA Free

Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TBC1D4 [p Ser588] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TBC1D4 [p Ser588] Antibody - Azide and BSA Free (NBP3-05451) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S588 of human TBC1D4 (NP_055647.2). LGSVD
• Modification: p Ser588
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBC1D4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for TBC1D4 [p Ser588] Antibody - Azide and BSA Free

• Akt substrate of 160 kDa
• AS160Acrg embryonic lethality minimal region ortholog
• DKFZp779C0666
• KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
• TBC1 domain family member 4
• TBC1 domain family, member 4

Background

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: TBC1D4