TBC1D4 [p Thr642] Antibody - Azide and BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TBC1D4 [p Thr642] Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: TBC1D4 [p Thr642] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT649 site of human TBC1D4 [p Thr642]. (Uniprot: Q8BYJ6)
• Modification: p Thr642
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBC1D4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry
  • Western Blot 1ug/ml
• Application Notes: This product is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~160kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

Cross-reactivity with TBC1D4 [p Thr642] from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for TBC1D4 [p Thr642] Antibody - Azide and BSA Free

• Akt substrate of 160 kDa
• AS160Acrg embryonic lethality minimal region ortholog
• DKFZp779C0666
• KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
• TBC1 domain family member 4
• TBC1 domain family, member 4

Background

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBC1D4
• Uniprot:
  • Q8BYJ6(Mouse)