T-bet/TBX21 Antibody (4B10)

Images

 
Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation ...read more
Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43298. Staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, Flow, Flow-IC, IP
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

T-bet/TBX21 Antibody (4B10) Summary

Immunogen
The immunogen for this antibody was TBet.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This 4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for T-bet/TBX21 Antibody (4B10)

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

The 4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP1-43298). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43298AF405
Alexa Fluor 488 Labeled NBP1-43298AF488
Alexa Fluor 647 Labeled NBP1-43298AF647
Alexa Fluor 700 Labeled NBP1-43298AF700
Allophycocyanin Labeled NBP1-43298APC
DyLight 350 Labeled NBP1-43298UV
DyLight 405 Labeled NBP1-43298V
DyLight 488 Labeled NBP1-43298G
DyLight 680 Labeled NBP1-43298FR
DyLight 755 Labeled NBP1-43298IR
PE Labeled NBP1-43298PE
PerCP Labeled NBP1-43298PCP
PerCP/Cy5.5 Labeled NBP1-42835

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Bioinformatics

Gene Symbol TBX21
Entrez