T-bet/TBX21 Antibody (4B10)


Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation ...read more
Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43298. Staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of ...read more

Product Details

Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, Flow, Flow-IC, IP
0.5 mg/ml

T-bet/TBX21 Antibody (4B10) Summary

The immunogen for this antibody was TBet.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This 4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified

Alternate Names for T-bet/TBX21 Antibody (4B10)

  • Tbet
  • T-bet
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM


The 4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP1-43298). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43298AF405
Alexa Fluor 488 Labeled NBP1-43298AF488
Alexa Fluor 647 Labeled NBP1-43298AF647
Alexa Fluor 700 Labeled NBP1-43298AF700
Allophycocyanin Labeled NBP1-43298APC
DyLight 350 Labeled NBP1-43298UV
DyLight 405 Labeled NBP1-43298V
DyLight 488 Labeled NBP1-43298G
DyLight 680 Labeled NBP1-43298FR
DyLight 755 Labeled NBP1-43298IR
PE Labeled NBP1-43298PE
PerCP Labeled NBP1-43298PCP
PerCP/Cy5.5 Labeled NBP1-42835

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Gene Symbol TBX21