T-bet/TBX21 Antibody (4B10)


Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation ...read more
Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43298. Staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, IP
0.5 mg/ml

T-bet/TBX21 Antibody (4B10) Summary

The immunogen for this antibody was TBet.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
This 4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for T-bet/TBX21 Antibody (4B10)

  • Tbet
  • T-bet
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM


The 4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, IHC, ICC
Species: Hu
Applications: WB, Simple Western, IHC, ICC
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: Flow, IHC, ICC
Species: Hu, Mu
Applications: ELISA(Cap), ELISA(Cap), ELISA(Det), ELISA(Det), Neut, ELISA(Sta), ELISA(Sta)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Species: Mu
Applications: Flow
Species: Hu
Applications: WB
Species: Hu
Applications: WB, IHC, ICFlow
Species: Hu
Applications: WB, ICC
Species: Hu, Mu
Applications: WB, Flow, Flow-IC, IP

Publications for T-bet/TBX21 Antibody (NBP1-43298) (0)

There are no publications for T-bet/TBX21 Antibody (NBP1-43298).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for T-bet/TBX21 Antibody (NBP1-43298) (0)

There are no reviews for T-bet/TBX21 Antibody (NBP1-43298). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP1-43298). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-43298AF405
Alexa Fluor (R) 488 Labeled NBP1-43298AF488
Alexa Fluor (R) 647 Labeled NBP1-43298AF647
Alexa Fluor (R) 700 Labeled NBP1-43298AF700
Allophycocyanin Labeled NBP1-43298APC
DyLight 350 Labeled NBP1-43298UV
DyLight 405 Labeled NBP1-43298V
DyLight 405LS Labeled NBP1-43298V3
DyLight 488 Labeled NBP1-43298G
DyLight 680 Labeled NBP1-43298FR
DyLight 755 Labeled NBP1-43298IR
PE Labeled NBP1-43298PE
PerCP Labeled NBP1-43298PCP
PerCP/Cy5.5 Labeled NBP1-42835

Additional T-bet/TBX21 Antibody (4B10) Products

Related Products by Gene

Bioinformatics Tool for T-bet/TBX21 Antibody (NBP1-43298)

Discover related pathways, diseases and genes to T-bet/TBX21 Antibody (NBP1-43298). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for T-bet/TBX21 Antibody (NBP1-43298)

Discover more about diseases related to T-bet/TBX21 Antibody (NBP1-43298).

Pathways for T-bet/TBX21 Antibody (NBP1-43298)

View related products by pathway.

PTMs for T-bet/TBX21 Antibody (NBP1-43298)

Learn more about PTMs related to T-bet/TBX21 Antibody (NBP1-43298).

Blogs on T-bet/TBX21

There are no specific blogs for T-bet/TBX21, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol TBX21

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-43298 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought