SYT11 Antibody - Azide and BSA Free

Western Blot: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Western blot analysis of extracts of various cell lines, using SYT11 antibody (A8198) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of mouse brain cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of rat brain cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SYT11 Antibody - Azide and BSA Free [NBP2-93989] - Immunofluorescence analysis of SH-5Y5Y cells using SYT11 Rabbit pAb (A8198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SYT11 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3). WSCCHQQAEKKQKNPPYKFIHMLKGISIYPETLSNKKKIIKVRRDKDGPGREGGRRNLLVDAAEAGLLSRDKDPRGPSSGSCIDQLPIKMDYGEELRSPITSLTPGESKTTSPSSPEEDVMLGSLTFSVDYNFPKKALVVTIQEAHGLPVMDDQTQGSDPYIKM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYT11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for SYT11 Antibody - Azide and BSA Free

• DKFZp781D015
• KIAA0080synaptotagmin 12
• MGC10881
• MGC17226
• synaptotagmin XISYT12
• synaptotagmin-11
• sytXI

Background

SYT11 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYT11