HIP1 Related Antibody (1E1)

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Western Blot: HIP1 Related Antibody (1E1) [NB300-206] - Analysis of HIP1 on HeLa whole cell extract.

Product Details

Summary
Reactivity Hu, Mu, Rt, BaSpecies Glossary
Applications WB, IHC, IP
Clone
1E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HIP1 Related Antibody (1E1) Summary

Immunogen
Human HIP1 Related-TALIN Homology Region (57 kDa). [UniProt# O75146]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIP1R
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
This HIP1 Related antibody is useful for Western blot, where a band is observed at ~120 kDa representing HIP1 Related protein. One lower molecular weight band 80 kDa may also be observed. Immunoprecipitation and Immunohistochemistry were reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59705)
HeLa Lysate (NB800-PC1)
HIPPI Lysate (NBL1-11860)
Publications
Read Publications using
NB300-206 in the following applications:

  • IHC
    1 publication
  • IP
    2 publications
  • WB
    4 publications

Reactivity Notes

Human, mouse, bacteria and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for HIP1 Related Antibody (1E1)

  • FLJ14000
  • HIP-12
  • HIP12HIP1-related protein
  • HIP3
  • huntingtin interacting protein 1 related
  • huntingtin interacting protein 12
  • Huntingtin-interacting protein 12
  • huntingtin-interacting protein 1-related protein
  • ILWEQ
  • KIAA0655FLJ27022
  • MGC47513

Background

Closely related to the clathrin-mediated endocytosis regulator HIP1, HIP1R is important in cytoskeletal and membrane traffic. HIP1R prolongs the half-life of growth factor receptors, and helps maintain diversity of adult tissues. This functionality seems to only be relevant in mature systems; whereas, in early development from embryo to juvenile stages, HIP1R does not seem to be critical unless other related proteins are affected. In the absence of HIP1R, growth and differentiation seems to occur without any major effects; however, when both HIP1R and HIP1 are deleted, kypholordosis and dwarfism occurs. This seems to indicate that HIP1 and HIP1R share similar roles in vivo, and can mostly compensate for the absence of each other.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIP1 Related Antibody (NB300-206)(4)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIP1R
Entrez
Uniprot