GAK Antibody (4C10) - Azide and BSA Free

Western Blot: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK expression in transfected 293T cell line by GAK monoclonal antibody (M01), clone 4C10.Lane 1: GAK transfected lysate (Predicted MW: 44 KDa).Lane 2: Non-transfected lysate.

Western Blot: GAK Antibody (4C10) [H00002580-M01] - GAK monoclonal antibody (M01), clone 4C10 Analysis of GAK expression in Hela S3 NE.

Immunoprecipitation: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK transfected lysate using anti-GAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GAK monoclonal antibody.

Sandwich ELISA: GAK Antibody (4C10) [H00002580-M01] - Detection limit for recombinant GST tagged GAK is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 4C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GAK Antibody (4C10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GAK (AAH08668, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQP
• Localization: Cytoplasm, perinuclear region. Golgi apparatus, trans Golgi network. Cell junction, focal adhesion. Localizes to the perinuclear area and to the trans Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
• Specificity: GAK - cyclin G associated kinase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GAK Antibody (4C10) - Azide and BSA Free

• Auxillin 2
• cyclin G associated kinase
• cyclin-G-associated kinase
• EC 2.7.11.1
• FLJ16629
• FLJ40395
• GAK
• MGC99654

Background

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAK
• Entrez:
  • 2580(Human)
• OMIM:
  • 602052(Human)
• Uniprot:
  • AAH08668(Human)