Syndecan-2/CD362 Antibodies

FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins." alt="NIH-3T3 mouse cell line was stained with Sheep Anti-Mouse Syndecan-2/CD362 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6585, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Syndecan-2/CD362 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Syndecan-2/CD362 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2965) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in glands. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in glands. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Syndecan-2/CD362 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB29651) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in glands. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965R) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965R) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965R) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />