Synaptotagmin 1 Antibody - Azide Free

Western Blot: Synaptotagmin 1 Antibody [NB300-739] - Western blot of rat cortex showing specific labeling of the ~65k Synaptotagmin protein.

• Reactivity: Mu, Rt, Hu, Bv, Ch, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Synaptotagmin 1 Antibody - Azide Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues specific to synaptotagmin 1 conjugated to KLH. Accession # P21707
• Specificity: Specific for endogenous levels of the ~65 kDa synaptotagmin I protein.
• Predicted Species: Human (100%), Chicken (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will react with human, mouse, bovine, chicken and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Synaptotagmin 1 Antibody - Azide Free

• DKFZp781D2042
• P65
• SVP65
• synaptotagmin Isynaptotagmin 1
• Synaptotagmin1
• Synaptotagmin-1
• SYT
• SYT1
• sytI

Background

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYT1
• Entrez:
  • 25716(Rat)
• Uniprot:
  • P21707(Rat)