STEAP1 Antibody (J2D2) - BSA Free

Immunocytochemistry/Immunofluorescence: STEAP1 Antibody (J2D2) [NBP1-07094] - staining of human LNCap cell colony with monoclonalanti-human STEAP1 antibody (J2D2) .

Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) [NBP1-07094] - Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Human breast cancer tissue (left) and prostate hyperplasia tissue (right).

Western Blot: STEAP1 Antibody (J2D2) [NBP1-07094] - The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-07094 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: LNcap cell lysate, Lane 2: Mouse Testis Tissue lysate

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: J2D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

STEAP1 Antibody (J2D2) - BSA Free Summary

• Description: Novus Biologicals Mouse STEAP1 Antibody (J2D2) - BSA Free (NBP1-07094) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human STEAP1 (1-70aa) purified from E. coli (NP_036581).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STEAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50SDS-PAGE 1:100-1:2000
  • Western Blot 1:1000-1:2000
• Application Notes: For IHC-P: Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for STEAP1 Antibody (J2D2) - BSA Free

• EC 1.16.1.-
• PRSS24
• PRSS24MGC19484
• six transmembrane epithelial antigen of the prostate 1
• six transmembrane epithelial antigen of the prostate
• Six-transmembrane epithelial antigen of prostate 1
• STEAP1
• STEAPmetalloreductase STEAP1

Background

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. Clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for STEAP1 Antibody (NBP1-07094). (Showing 1 - 2 of 2 FAQ).

1. Can I received overnight shipping on NBP1-07094 (anti-STEAP1 Mouse IgG2b antibody)?
   • Unfortunately, NBP1-07094 is currently available in 6 to 8 business days, as is stated on our website. I apologize for the inconvenience. To view the price and availability of our products, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information on the product's datasheet.
2. May any of your anti human-STEAP1 Abs be used for flow cytometry, with a secondary anti-mouse antibody? E.g. J2D2 or 4F6-1F3?
   • Unfortunately, none of our STEAP1 antibodies have been validated in flow cytometry yet. This does not mean they will not work in FLOW, we just cannot guarantee its use in FLOW application because we have not tested them in this application in our lab yet. However, if you would like to try one of these antibodies in FLOW, you would be eligible for our Innovator's Reward, whereby if you share your results with us, we will reward you with a voucher for 100% of the purchase price - see here for more details: Innovator's Reward program. Technically, STEAP1 (J2D2) NBP1-07094 and STEAP1 (4F6-1F3) H00026872-M01 are the two antibodies that have more chances of success because of proven compatibility with IF labeled secondary antibodies as they have already been validated in ICC-IF.

Bioinformatics

• Gene Symbol: STEAP1
• Entrez:
  • 26872(Human)
• Uniprot:
  • NP_036581()