STAMP2/STEAP4 Antibody

Images

 
Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - HEK293 overexpressing Dudulin4 and probed with (mock transfection in first lane).
Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - (0.3ug/ml) Analysis of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoprecipitation: STAMP2/STEAP4 Antibody [NB100-68162] - Analysis of STEAP4 in MES13 cells using anti-STEAP4 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

STAMP2/STEAP4 Antibody Summary

Immunogen
Peptide with sequence CVDNTLTRIRQGWERN corresponding to C-Terminus according to NP_078912.2.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STEAP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 50-55 kDa band observed in human adipose lysates (calculated MW of 52.0 kDa band according to NP_078912.2). In transfected HEK293 transiently expressing human Dudulin4 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IP reactivity per customer review.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
STAMP2/STEAP4 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-68162 in the following applications:

Publications
Read Publications using
NB100-68162 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in (PMID:29078383).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STAMP2/STEAP4 Antibody

  • EC 1.16.1
  • EC 1.16.1.-
  • FLJ23153
  • Six-transmembrane epithelial antigen of prostate 4
  • SixTransMembrane protein of prostate 2
  • STAMP2
  • STAMP2DKFZp666D049
  • STEAP family member 4
  • STEAP4
  • TIARP
  • TIARPsix transmembrane prostate protein 2
  • TNFAIP9
  • Tumor necrosis factor, alpha-induced protein 9metalloreductase STEAP4
  • tumor necrosis-alpha-induced adipose-related protein

Background

STEAP4 is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. It is similar to two other members of the STEAP family, STEAP2 and STEAP3. STEAP4 promotes the reduction of both iron (Fe3+ to Fe2+) and copper (Cu2+ to Cu1+). STEAP4 is highly expressed in placenta, lung, heart and prostate tissues. Furthermore, it is over expressed in prostate cancer cells compared to normal prostate cells. STEAP4 may have a role in cell proliferation and prostrate cancer progression since over expression of STEAP4 in prostate cells significantly increases cell growth and colony formation. At least two isoforms of this protein are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP, PEP-ELISA

Publications for STAMP2/STEAP4 Antibody (NB100-68162)(3)

Review for STAMP2/STEAP4 Antibody (NB100-68162) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-68162:
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IP
(1)
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Images Ratings Applications Species Date Details
Immunoprecipitation STAMP2/STEAP4 NB100-68162
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5
reviewed by:
Verified Customer
IP Mouse 05/11/2015
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Summary

ApplicationImmunoprecipitation
Sample TestedMES13 cells
SpeciesMouse

Blocking

Blocking Details5% non-fat milk

Primary Anitbody

Dilution Ratio1:50, overnight incubation at 4C

Details

Detection NotesSee PMID 25817898
Immunoprecipitation Detailsprotein A/protein G magnetic beads

Comments

CommentsSee PMID 25817898 for details regarding the protocol.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAMP2/STEAP4 Antibody (NB100-68162) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
05/11/2015
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol STEAP4
Entrez
Uniprot