PSGR Overexpression Lysate (Native)

Western Blot: PSGR Overexpression Lysate (Adult Normal) [NBL1-13961] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSGR.

• Reactivity: Hu
• Applications: WB

PSGR Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC204752 and based on accession number NM_030774.
• Epitope: C-terminal DDK tag
• Specificity: Homo sapiens olfactory receptor, family 51, subfamily E, member 2 (OR51E2), mRNA.
• Gene: OR51E2

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 35.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PSGR Overexpression Lysate (Native)

• HPRAJ
• Olfactory Receptor OR11-16
• olfactory receptor, family 51, subfamily E, member 2
• olfactory receptor, family 51, subfamily E, member 3 pseudogene
• olfactory receptor, family 52, subfamily A, member 2
• OR51E2
• OR51E3P
• OR52A2
• prostate specific G-protein coupled receptor
• Prostate-specific G-protein coupled receptor
• PSGR
• PSGRolfactory receptor 51E2

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Review for PSGR Lysate (NBL1-13961) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: sean taheri WB Human 05/26/2010

Summary

• Application: Western Blot
• Sample Tested: PSGR Lysate, Sample Amount: 20 ug
• Species: Human
• Comments: visible bands were detected at expected size of protein of interest

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: 4 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: LS-A20207, Ab13383, SC69543, Secondary Ab Dilution Ratio: 1:1000
• Secondary Manufacturer Cat#: LS-A20207, Ab13383, SC69543

Details

• Detection Notes: Detection Method: ECL, Exposure Time:10min, Pos Ctrl:PSGR lystae, Neg Ctrl: MSCf-7 whole cell lysate, Specific Bands: 45kDa

Comments

• Comments: visible bands were detected at expected size of protein of interest

Bioinformatics

• Gene Symbol: OR51E2