PSGR Overexpression Lysate (Native)

Images

 
Western Blot: PSGR Overexpression Lysate (Adult Normal) [NBL1-13961] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSGR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PSGR Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204752 and based on accession number NM_030774.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens olfactory receptor, family 51, subfamily E, member 2 (OR51E2), mRNA.
Gene
OR51E2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBL1-13961 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PSGR Overexpression Lysate (Native)

  • HPRAJ
  • Olfactory Receptor OR11-16
  • olfactory receptor, family 51, subfamily E, member 2
  • olfactory receptor, family 51, subfamily E, member 3 pseudogene
  • olfactory receptor, family 52, subfamily A, member 2
  • OR51E2
  • OR51E3P
  • OR52A2
  • prostate specific G-protein coupled receptor
  • Prostate-specific G-protein coupled receptor
  • PSGR
  • PSGRolfactory receptor 51E2

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PSGR Lysate (NBL1-13961) (0)

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Review for PSGR Lysate (NBL1-13961) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBL1-13961:
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(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
sean taheri
WB Human 05/26/2010
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Summary

ApplicationWestern Blot
Sample TestedPSGR Lysate, Sample Amount: 20 ug
SpeciesHuman
Commentsvisible bands were detected at expected size of protein of interest

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: LS-A20207, Ab13383, SC69543, Secondary Ab Dilution Ratio: 1:1000
Secondary Manufacturer Cat#LS-A20207, Ab13383, SC69543

Details

Detection NotesDetection Method: ECL, Exposure Time:10min, Pos Ctrl:PSGR lystae, Neg Ctrl: MSCf-7 whole cell lysate, Specific Bands: 45kDa

Comments

Commentsvisible bands were detected at expected size of protein of interest

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FAQs for PSGR Lysate (NBL1-13961) (0)

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Recent Reviews

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sean taheri
05/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol OR51E2
Entrez