PSGR Overexpression Lysate

Images

 
Western Blot: PSGR Overexpression Lysate (Adult Normal) [NBL1-13961] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSGR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PSGR Overexpression Lysate Summary

Description

PSGR Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204752

Accession#: NM_030774

Protein Tag: C-MYC/DDK
Gene
OR51E2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
35.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBL1-13961 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PSGR Overexpression Lysate

  • HPRAJ
  • Olfactory Receptor OR11-16
  • olfactory receptor, family 51, subfamily E, member 2
  • olfactory receptor, family 51, subfamily E, member 3 pseudogene
  • olfactory receptor, family 52, subfamily A, member 2
  • OR51E2
  • OR51E3P
  • OR52A2
  • prostate specific G-protein coupled receptor
  • Prostate-specific G-protein coupled receptor
  • PSGR
  • PSGRolfactory receptor 51E2

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PSGR Lysate (NBL1-13961) (0)

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Review for PSGR Lysate (NBL1-13961) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBL1-13961:
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WB
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  3
reviewed by:
Verified Customer
WB Human 05/26/2010
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Summary

ApplicationWestern Blot
Sample TestedPSGR Lysate, Sample Amount: 20 ug
SpeciesHuman

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Verified Customer
05/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol OR51E2