STAT5A [Asym-dimethyl Arg31] Antibody - BSA Free

Western Blot: STAT5A [Asym-dimethyl Arg31] Antibody [NBP2-44185] - Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

STAT5A [Asym-dimethyl Arg31] Antibody - BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: STAT5A [Asym-dimethyl Arg31] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT. (Uniprot: P42229)
• Modification: Asym-dimethyl Arg31
• Localization: Cytoplasm, Nucleus (upon phosphorylation at Tyr694)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Western Blot 1:500
• Application Notes: This product has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for STAT5A [Asym-dimethyl Arg31] Antibody - BSA Free

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A
• Uniprot:
  • P42229(Human)