STAT5A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: STAT5A Antibody [NBP2-20505] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505] - Paraffin-embedded mouse colon. STAT5A antibody [C2C3], C-term dilution: 1:250.

Western Blot: STAT5A Antibody [NBP2-20505] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505] - araffin-embedded mouse fore brain. STAT5A antibody [C2C3], C-term dilution: 1:250.

Whole cell extract (22.5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (NBP2-20505) diluted at 1:2000.

Whole cell extract (12.7 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (NBP2-20505) diluted at 1:5000.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

STAT5A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Rat (93%), Porcine (92%), Bovine (93%), Rhesus Macaque (97%), Sheep (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT5A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for STAT5A Antibody - BSA Free

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STAT5A
• Uniprot:
  • P42229(Human)