STAT3 [p Tyr705] Antibody (805435)

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 805435
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT3 [p Tyr705] Antibody (805435) Summary

• Immunogen: Phosphopeptide containing the human STAT3 Y705 site
• Modification: p Tyr705
• Specificity: Detects human STAT3 when phosphorylated at Y705.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: STAT3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT3 [p Tyr705] Antibody (805435)

• Acute-phase response factor
• APRFMGC16063
• DNA-binding protein APRF
• FLJ20882
• HIES
• signal transducer and activator of transcription 3 (acute-phase responsefactor)
• signal transducer and activator of transcription 3
• STAT3

Background

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT3 Antibody (MAB4607)(5)

Publications using MAB4607

• Bermejo-Álvarez, I;Pérez-Baos, S;Gratal, P;Medina, JP;Largo, R;Herrero-Beaumont, G;Mediero, A; Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia International journal of molecular sciences 2023-08-24 [PMID: 37685986] (Western Blot, Rabbit)
• Zhao, X;Li, N;Yang, N;Mi, B;Dang, W;Sun, D;Ma, S;Nian, H;Wei, R; Thymosin ?4 Alleviates Autoimmune Dacryoadenitis via Suppressing Th17 Cell Response Investigative ophthalmology & visual science 2023-08-01 [PMID: 37531112] (Western Blot, Rabbit)
• Sandra Pérez-Baos, Paula Gratal, Juan I. Barrasa, Ana Lamuedra, Olga Sánchez-Pernaute, Gabriel Herrero-Beaumont et al. Inhibition of pSTAT1 by tofacitinib accounts for the early improvement of experimental chronic synovitis Journal of Inflammation 12/1/2019 [PMID: 30728752]
• H Xu, Y Zhou, W Li, B Zhang, H Zhang, S Zhao, P Zheng, H Wu, J Yang Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer Oncol Lett, 2018-04-11;15(6):9142-9150. 2018-04-11 [PMID: 29844821] (Western Blot, Human)
• RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Sci Rep, 2017-07-24;7(1):6311. 2017-07-24 [PMID: 28740214] (Western Blot, Rabbit)

Bioinformatics

• Gene Symbol: STAT3