STAM-1 Antibody (HL1637)

STAM-1 antibody [HL1637] detects STAM-1 protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STAM-1 stained by STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:500.Blue: Fluoroshield with DAPI .

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) 293T whole cell extracts (10 | 10 | 20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM-1 antibody [HL1637] (NBP3-48660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: HL1637
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

STAM-1 Antibody (HL1637) Summary

• Description: Novus Biologicals Rabbit STAM-1 Antibody (HL1637) (NBP3-48660) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human STAM-1
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STAM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:25000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for STAM-1 Antibody (HL1637)

• DKFZp686J2352
• HSE1 Homolog
• signal transducing adapter molecule 1
• signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• STAM
• STAM1
• STAM-1
• STAM1STAM-1

Background

STAM was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAM