Human ST2/IL-33R ELISA Kit (Colorimetric)

ELISA: Human IL1RL1 ELISA Kit (Colorimetric) [NBP2-60069] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Human ST2/IL-33R ELISA Kit (Colorimetric) [NBP2-60069] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 0.312 ng/mL - 20 ng/mL
• Sensitivity: 0.078 ng/mL

Human ST2/IL-33R ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IL1RL1. No significant cross-reactivity or interference between human IL1RL1 and analogues was observed.
• Standard Curve Range: 0.312 ng/mL - 20 ng/mL
• Sensitivity: 0.078 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL1RL1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ST2/IL-33R ELISA Kit (Colorimetric)

• DER4
• DER4ST2growth stimulation-expressed
• Fit-1
• IL-1 R4
• IL1R4
• IL-1R4
• IL-1RL1
• IL-33 R
• IL33R
• IL-33R
• interleukin 1 receptor-like 1
• interleukin 1 receptor-related protein
• interleukin-1 receptor-like 1
• Ly84
• MGC32623
• Protein ST2
• ST2
• ST2L
• ST2V
• T1
• T1homolog of mouse growth stimulation-expressed

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1RL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1RL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1RL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1RL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL1RL1