ST2/IL-33R Antibody [Unconjugated]

Recombinant Human IL‑33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically1-6 μg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ST2/IL-33R Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
  Ser27-Arg332
  Accession # P14719
• Specificity: Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il1rl1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1-6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST2/IL-33R Antibody [Unconjugated]

• DER4
• DER4ST2growth stimulation-expressed
• Fit-1
• IL-1 R4
• IL1R4
• IL-1R4
• IL-1RL1
• IL-33 R
• IL33R
• IL-33R
• interleukin 1 receptor-like 1
• interleukin 1 receptor-related protein
• interleukin-1 receptor-like 1
• Ly84
• MGC32623
• Protein ST2
• ST2
• ST2L
• ST2V
• T1
• T1homolog of mouse growth stimulation-expressed

Background

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).

1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
4. Schmitz, J. et al. (2005) Immunity 23:479.
5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
9. Palmer, G. et al. (2008) Cytokine 42:358.
10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST2/IL-33R Antibody (AF1004)(11)

Publications using AF1004

• M Liu, J Huang, T Liu, J Yuan, C Lv, Z Sha, C Wu, W Jiang, X Liu, M Nie, Y Chen, S Dong, Y Qian, C Gao, Y Fan, D Wu, R Jiang Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice Acta neuropathologica communications, 2023-04-07;11(1):61. 2023-04-07 [PMID: 37024941] (In Vivo, Mouse)
• Jaiswal AK, Makhija S, Stahr N Et al. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation Immunobiology 8/5/2020 [PMID: 32747016]
  
  Details:
  Citation using the PE version of this antibody.
• Yanyan Zeng, Yu Shi, Hongrui Zhan, Wei Liu, Guiyuan Cai, Haili Zhong et al. Reduction of Silent Information Regulator 1 Activates Interleukin-33/ST2 Signaling and Contributes to Neuropathic Pain Induced by Spared Nerve Injury in Rats Frontiers in Molecular Neuroscience 2020-02-12 [PMID: 32116550]
• Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice J Innate Immun, 2018-01-27;0(0):. 2018-01-27 [PMID: 29393212] (Western Blot, Mouse)
• Xiaoqing Zhu, Yinghua Zhao, Yuxue Jiang, Tianxue Qin, Jintong Chen, Xiao Chu et al. Dectin-1 signaling inhibits osteoclastogenesis via IL-33-induced inhibition of NFATc1 Oncotarget 2017-08-08 [PMID: 28881817]
• Dos Santos JC, Grund LZ, Seibert CS et al. Stingray venom activates IL-33 producing cardiomyocytes, but not mast cell, to promote acute neutrophil-mediated injury Sci Rep 8/11/2017 [PMID: 28801624]
• Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3. J Immunol, 2014-01-24;192(4):1972-81. 2014-01-24 [PMID: 24465013] (Neutralization, Mouse)
• Mizutani N, Nabe T, Yoshino S Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice. Immunology, 2013-06-01;139(2):205-18. 2013-06-01 [PMID: 23323935] (Neutralization, Mouse)
• Hsu CL, Neilsen CV, Bryce PJ IL-33 is produced by mast cells and regulates IgE-dependent inflammation. PLoS ONE, 2010-08-03;5(8):e11944. 2010-08-03 [PMID: 20689814] (Neutralization, Mouse)
• Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. J. Immunol., 2008-02-15;180(4):2322-8. 2008-02-15 [PMID: 18250441] (IHC-Fr, Mouse)
• Huang X, Du W, Barrett RP, Hazlett LD ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis. Invest. Ophthalmol. Vis. Sci., 2007-10-01;48(10):4626-33. 2007-10-01 [PMID: 17898286] (Western Blot, Mouse)

Review for ST2/IL-33R Antibody (AF1004) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse
• Lot: PO number: P00115619

Bioinformatics

• Gene Symbol: Il1rl1
• Uniprot:
  • P14719()