SREC-I/SCARF1 Antibody

Western Blot: SREC-I/SCARF1 Antibody [NB100-1456] - Human Heart lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Antibody at 0.3 ug/mL, 1 hr incubation. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SREC-I/SCARF1 Antibody Summary

• Description: Novus Biologicals Goat SREC-I/SCARF1 Antibody (NB100-1456) is a polyclonal antibody validated for use in WB and ELISA. Anti-SREC-I/SCARF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SELDPKGQHVC corresponding to internal region according to NP_003684.2, NP_663325.1, NP_663327.2.
• Epitope: Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 protein.
• Localization: Type I membrane protein
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_003684.2; NP_663325.1; NP_663327.2). Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCARF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1.0 ug/mL
• Application Notes: WB: Approx. 85-90 kDa band observed in human heart lysates (calculated MW of 87.4 kDa band according to NP_003684). An additional faint band of 28 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SREC-I/SCARF1 Antibody

• Acetyl LDL receptor
• KIAA0149endothelial cells scavenger receptor
• MGC47738
• SCARF1
• scavenger receptor class F, member 1
• Scavenger receptor expressed by endothelial cells 1
• SREC1
• SRECI
• SREC-I
• SRECscavenger receptor expressed by endothelial cells
• SR-F1

Background

The protein recognised by this antibody is a scavenger receptor that is expressed in endothelial cells and mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Transcription factors ZNF444/EZF-2 and SP1 are found to be responsible for the regulation of this gene. Five alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SREC-I/SCARF1 Antibody (NB100-1456)(1)

Publication using NB100-1456

• Adachi H, Tsujimoto M, Arai H, Inoue K. Expression cloning of a novel scavenger receptor from human endothelial cells. J Biol Chem 1997-12-12 [PMID: 9395444]

Bioinformatics

• Gene Symbol: SCARF1
• Entrez:
  • 8578(Human)
• Uniprot:
  • NP_003684(Human)