SREBP1 Antibody (2A4) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa
• Applications: WB, IHC, KO
• Clone: 2A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

SREBP1 Antibody (2A4) [Alexa Fluor™ Plus 680] Summary

• Immunogen: 6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SREBF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Frozen
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SREBP1 Antibody (2A4) [Alexa Fluor™ Plus 680]

• bHLHd1
• Class D basic helix-loop-helix protein 1
• SREBF1
• SREBP 1c
• SREBP1
• SREBP-1
• SREBP1bHLHd1SREBP-1c
• sterol regulatory element binding protein-1
• sterol regulatory element binding transcription factor 1
• sterol regulatory element-binding protein 1
• Sterol regulatory element-binding transcription factor 1

Background

SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. SREBPs may also play some role in the apoptotic processes.

SREBP1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SREBF1