SRD5A2 Antibody (SR1475) - BSA Free

Western Blot: SRD5A2 Antibody (SR1475) [NBP3-22258] - Western blot analysis using NBP3-22258 in PC3 cell lysate.

• Reactivity: Hu, Rt
• Applications: WB, IP
• Clone: SR1475
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRD5A2 Antibody (SR1475) - BSA Free Summary

• Description: Novus Biologicals Rabbit SRD5A2 Antibody (SR1475) - BSA Free (NBP3-22258) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human SRD5A2 (Uniprot #: P31213)
• Specificity: Detects endogenous levels of total SRD5A2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRD5A2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SRD5A2 Antibody (SR1475) - BSA Free

• 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
• 5 alpha-SR2
• EC 1.3.99.5
• MGC138457
• S5AR 2
• SR type 2
• SRD5A2
• Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
• steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
• Type II 5-alpha reductase

Background

SRD5A2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRD5A2