SPTLC1 Antibody

Western Blot:[NBP3-30449]-Detection of human and mouse SPTLC1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SPTLC1 antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. SPTLC1 was also immunoprecipitated by rabbit anti-SPTLC1 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SPTLC1 Antibody Summary

• Description: Novus Biologicals Rabbit SPTLC1 Antibody (NBP3-30449) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 50 and 100
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTLC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for SPTLC1 Antibody

• EC 2.3.1.50
• hereditary sensory neuropathy, type 1
• HSAN1
• LBC1
• LCB 1
• LCB1
• LCB1HSAN
• Long chain base biosynthesis protein 1
• MGC14645
• serine C-palmitoyltransferase
• serine palmitoyltransferase 1
• serine palmitoyltransferase, long chain base subunit 1
• Serine-palmitoyl-CoA transferase 1
• SPT 1
• SPT1
• SPT1HSN1
• SPTI
• SPTLC1

Background

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTLC1