SPTLC1 Antibody

Images

 
Western Blot: SPTLC1 Antibody [NBP1-59643] - HepG2 cell lysate, concentration 0.5ug/ml.
Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-59643] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: SPTLC1 Antibody [NBP1-59643] - SPTLC1 levels in human neuroblastoma cells. Cells were untreated or treated with various drugs. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SPTLC1 Antibody Summary

Immunogen
Synthetic peptides corresponding to SPTLC1(serine palmitoyltransferase, long chain base subunit 1) The peptide sequence was selected from the middle region of SPTLC1. Peptide sequence ICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLI.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPTLC1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against SPTLC1 and was validated on Western Blot and immunohistochemistry-paraffin
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for SPTLC1 Antibody

  • EC 2.3.1.50
  • hereditary sensory neuropathy, type 1
  • HSAN1
  • LBC1
  • LCB 1
  • LCB1HSAN
  • Long chain base biosynthesis protein 1
  • MGC14645
  • serine C-palmitoyltransferase
  • serine palmitoyltransferase 1
  • serine palmitoyltransferase, long chain base subunit 1
  • Serine-palmitoyl-CoA transferase 1
  • SPT 1
  • SPT1HSN1
  • SPTI

Background

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1.Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPTLC1
Uniprot