SPRY4 Antibody

Images

 
Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded mouse duodenum. SPRY4 antibody diluted at 1:500.
Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded U87 xenograft. SPRY4 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SPRY4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SPRY4. The exact sequence is proprietary.
Localization
Cytoplasm; Cell projection; ruffle membrane; Peripheral membrane protein; Cytoplasmic side
Predicted Species
Rat (94%), Sheep (94%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPRY4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPRY4 Antibody

  • protein sprouty homolog 4
  • sprouty homolog 4 (Drosophila)
  • SPRY4
  • spry-4

Background

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SPRY4 Antibody (NBP2-20479)

Discover related pathways, diseases and genes to SPRY4 Antibody (NBP2-20479). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol SPRY4
Entrez
Uniprot