SPRY1 Antibody (3H4) - Azide and BSA Free

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - SPRY1 monoclonal antibody (M01), clone 3H4 Analysis of SPRY1 expression in HepG2.

Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.Lane 1: SPRY1 transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.

ELISA: SPRY1 Antibody (3H4) [H00010252-M01] - Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPRY1 Antibody (3H4) - Azide and BSA Free Summary

• Immunogen: SPRY1 (NP_005832, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPI
• Specificity: SPRY1 - sprouty homolog 1, antagonist of FGF signaling (Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPRY1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPRY1 Antibody (3H4) - Azide and BSA Free

• Drosophila, homolog of, 1 (antagonist of FGF signaling)
• sprouty homolog 1, antagonist of FGF signaling (Drosophila)
• SPRY1
• spry-1

Background

Sprouty may function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPRY1 Antibody (H00010252-M01)(3)

Publications using H00010252-M01

• Zhang L, Deng J, Pan Q et al. Targeted methylation sequencing reveals dysregulated Wnt signaling in Parkinson disease. J Genet Genomics 2016-05-13 [PMID: 27692691]
• Mekkawy AH, Pourgholami MH, Morris DL. Human Sprouty1 suppresses growth, migration, and invasion in human breast cancer cells. Tumour Biol. 2014-02-08 [PMID: 24510305]
• Moghaddam SM, Amini A, Wei AQ et al. Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines. J Oncol. 2012-11-29 [PMID: 23251157]

Bioinformatics

• Gene Symbol: SPRY1
• Entrez:
  • 10252(Human)
• OMIM:
  • 602465(Human)
• Uniprot:
  • NP_005832(Human)