Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-30856] - Western blot analysis using Sphingosine Kinase 1/SPHK1 antibody at a dilution of 1:400 on MDBK cell lysate using Novus Biologicals' mouse anti-rabbit HRP secondary antibody at 1:75,000.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sphingosine Kinase 1/SPHK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Sphingosine Kinase 1/SPHK1 Antibody - BSA Free (NBP3-30856) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rabbits were immunized with a synthetic peptide derived from the C-terminal of human Sphingosine Kinase 1/SPHK1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot : 1:400
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1