Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free

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Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of endometrial cancer tissues using SK2 mouse mAb with DAB staining.
Flow Cytometry: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of bladder cancer tissues using SK2 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
ELISA: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
9C5E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free Summary

Immunogen
Synthesized peptide of human Sphingosine Kinase 2/SPHK2 (AA: 36-52).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPHK2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot
Application Notes
This antibody is Cytof ready. Use in Western Blot reported in scientific literature (PMID:32146198).
Theoretical MW
69.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-52567 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free

  • EC 2.7.1.91
  • SK 2
  • SK2
  • sphingosine kinase 2
  • sphingosine kinase type 2 isoform
  • SPHK2
  • SPK 2
  • SPK2

Background

SPHK2 catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sphingosine Kinase 2/SPHK2 Antibody (NBP2-52567)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPHK2