Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of endometrial cancer tissues using SK2 mouse mAb with DAB staining.

Flow Cytometry: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).

Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of bladder cancer tissues using SK2 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.

ELISA: Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) [NBP2-52567] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 9C5E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free Summary

• Immunogen: Synthesized peptide of human Sphingosine Kinase 2/SPHK2 (AA: 36-52).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPHK2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot
• Application Notes: This antibody is Cytof ready. Use in Western Blot reported in scientific literature (PMID:32146198).
• Theoretical MW: 69.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Sphingosine Kinase 2/SPHK2 Antibody (9C5E1) - BSA Free

• EC 2.7.1.91
• SK 2
• SK2
• sphingosine kinase 2
• sphingosine kinase type 2 isoform
• SPHK2
• SPK 2
• SPK2

Background

SPHK2 catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sphingosine Kinase 2/SPHK2 Antibody (NBP2-52567)(1)

Publication using NBP2-52567

• Chen L, Xia Y, Lu J et Al. A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway Ecotoxicol. Environ. Saf. 2020-05-01 [PMID: 32146198] (WB, Human)

Bioinformatics

• Gene Symbol: SPHK2