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HAF016). A specific band was detected for Sphingosine Kinase 1/SPHK1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox" target="_blank">
MAB5536) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Sphingosine Kinase 1/SPHK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5536) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox" target="_blank">
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]
![Western Blot Sphingosine Kinase 1/SPHK1 Antibody [Unconjugated]](http://images.novusbio.com/images/af5536_human-sphingosine-kinase-1-sphk1-affinity-purified-pab-western-blot-639195800400798304.jpg)
![Immunocytochemistry Sphingosine Kinase 1/SPHK1 Antibody [Unconjugated]](http://images.novusbio.com/images/af5536_human-sphingosine-kinase-1-sphk1-affinity-purified-pab-immunocytochemistry-639195800781236825.jpg)
![Immunohistochemistry Sphingosine Kinase 1/SPHK1 Antibody [Unconjugated]](http://images.novusbio.com/images/af5536_human-sphingosine-kinase-1-sphk1-affinity-purified-pab-immunohistochemistry-639195801091924126.jpg)
![ELISA Sphingosine Kinase 1/SPHK1 Antibody [Unconjugated]](http://images.novusbio.com/images/af5536_human-sphingosine-kinase-1-sphk1-affinity-purified-pab-elisa-639195801669882855.jpg)
