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S1P2/EDG-5/S1PR2 Antibody

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Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.
Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and ...read more
Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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S1P2/EDG-5/S1PR2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.
Predicted Species
Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Use in IHC-P reported in scientific literature (PMID 25621765)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-26691 in the following applications:
Publications
Read Publications using
NBP2-26691 in the following applications:

  • IHC-P
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody

  • AGR16
  • EDG5
  • EDG-5
  • endothelial differentiation G-protein coupled receptor 5
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
  • GPCR13
  • GPCR18
  • H218
  • LPB2
  • S1P receptor 2
  • S1P receptor EDG5
  • S1P receptor Edg-5
  • S1P2
  • S1PR2
  • sphingosine 1-phosphate receptor 2
  • sphingosine 1-phosphate receptor Edg-5
  • sphingosine-1-phosphate receptor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.
Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.
Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.
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Western Blot: S1P5/EDG-8 Antibody [NBP2-24712] - Analysis of EDG8 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse, 4) rat using NBP2-24712 at 5 ug/ml, 2 ug/ml and 2 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using NBP2-24712 at 5 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt, Ca, Pm, Xp
Applications: WB, IHC, IHC-P
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Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.
Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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(1 Review)
(3 Publications)
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
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Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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(1 Review)
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(1 Publication)
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(2 Publications)
Species: Hu, Mu
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Western Blot: BUD31 Antibody [NBP1-84010] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: BUD31 Antibody [NBP1-84010] - Staining of human cell line U-2 OS shows localization to nucleus & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BUD31 Antibody [NBP1-84010] - Staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB
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NB100-91273
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Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
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[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(9 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Recombinant Human
Signal Peptide Peptid ...
H00081502-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: WB
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Publications for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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Review for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human .

Reviews using NBP2-26691:
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Images Ratings Applications Species Date Details
Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
Enlarge 		4
reviewed by:
Dimitra Kalamida
 IF Human 07/11/2018
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Summary

ApplicationImmunofluorescence
Sample TestedC42B prostate cell line,PC3 prostate cancer cell line
SpeciesHuman
Lot01013168A-04

Comments

Comments1:200 dilution of the antibody used

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Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
Enlarge
 Dimitra Kalamida
07/11/2018
Application: IF
Species: Human
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Bioinformatics

Gene Symbol S1PR2
Uniprot