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S1P2/EDG-5/S1PR2 Antibody

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Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.
Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and ...read more
Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

S1P2/EDG-5/S1PR2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.
Predicted Species
Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID 25621765)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-26691 in the following applications:
Publications
Read Publications using
NBP2-26691 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody

  • AGR16
  • EDG5
  • EDG-5
  • endothelial differentiation G-protein coupled receptor 5
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
  • GPCR13
  • GPCR18
  • H218
  • LPB2
  • S1P receptor 2
  • S1P receptor EDG5
  • S1P receptor Edg-5
  • S1P2
  • S1PR2
  • sphingosine 1-phosphate receptor 2
  • sphingosine 1-phosphate receptor Edg-5
  • sphingosine-1-phosphate receptor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human colon cancer using MBTPS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human stomach using MBTPS1 antibody at dilution of 1:100 (40x lens).
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HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
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S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.
Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.
Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.
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Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
Immunohistochemistry-Paraffin: S1P5/EDG-8 Antibody [NBP2-24712] - Staining of normal human brain using NBP2-24712 at 5 ug/ml.
Western Blot: S1P5/EDG-8 Antibody [NBP2-24712] - Analysis of EDG8 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse, 4) rat using NBP2-24712 at 5 ug/ml, 2 ug/ml and 2 ug/ml.
(1 Publication)
Species: Ca, Hu, Mu, Pm, Rt, Xp
Applications: IHC, IHC-P, WB
LPAR1/LPA1/EDG-2
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Simple Western: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Simple Western lane view shows a specific band for EDG2 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.
Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(1 Review)
(5 Publications)
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Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
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Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.
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(1 Review)
(3 Publications)
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Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X.
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ELISA: Lipoprotein a Antibody (4H1) [NBP2-37477] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: Lipoprotein a Antibody (4H1) [NBP2-37477] - Western blot analysis using LPA mAb against human LPA recombinant protein. (Expected MW is 34.1 kDa)
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Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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AKT [p Ser473]
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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.
Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).
(1 Review)
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S1P4/EDG-6
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Immunohistochemistry-Paraffin: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of Edg6/S1P4 in paraffin-embedded formalin-fixed mouse RAW cells using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: S1P4/EDG-6 Antibody [NBP2-24500] - Analysis of EDG6 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW using NBP2-24500 at 4.0 ug/ml.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: LPAR2/LPA2/EDG-4 Antibody [NLS1017] - Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human heart, myocardial infarct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
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NBP1-84010
Western Blot: BUD31 Antibody [NBP1-84010] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: BUD31 Antibody [NBP1-84010] - Staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Immunocytochemistry/Immunofluorescence: BUD31 Antibody [NBP1-84010] - Staining of human cell line U-2 OS shows localization to nucleus & microtubules. Antibody staining is shown in green.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
LPAR2/LPA2/EDG-4
Antibody
AF6396
Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB
RhoA Antibody
NB100-91273
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
(1 Review)
(4 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(5 Reviews)
(39 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Signal Peptide
Peptidase Antibody
NBP1-92389
Western Blot: Signal Peptide Peptidase Antibody [NBP1-92389] - Analysis in control (vector only transfected HEK293T lysate) and HM13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Signal Peptide Peptidase Antibody [NBP1-92389] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
Species: Hu
Applications: IHC, IHC-P, WB
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Publications for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


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ICC/IF
(2)
IHC
(2)
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(1)
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Showing Publications 1 - 5 of 5.
Publications using NBP2-26691 Applications Species
Xiao P, Gu J, Xu W et al. RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction Autophagy Mar 9 2022 [PMID: 35263212] (IHC, Rat) IHC Rat
Pan G, Liao M, Dai Y et al. Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture Frontiers in Cardiovascular Medicine Dec 17 2021 [PMID: 34977175] (IHC, WB, ICC/IF, Human) IHC, WB, ICC/IF Human
Tran HB, Maiolo S, Harper R Et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels Cell biology international Aug 4 2021 [PMID: 34347342] (ICC/IF, Rat) ICC/IF Rat
Gu Y, Shea J, Slattum G et al. Defective apical extrusion signaling contributes to aggressive tumor hallmarks Elife. 2015 Feb 11 [PMID: 25621765] (IHC-P, Human)

Details:
S1P2/EDG-5/S1PR2 antibody used for IHC-P staining of formalin-fixed, paraffin-embedded tissue sections of Human Pancreatic Ductal Adenocarcinomas /PDACs - heat induced antigen retrieval with 10 mM sodium citrate for 20 minutes, blocking with 5% BSA/0.5% Tween-20 in PBS for 4 hours, primary incubated for ON at 4C, detection with Alexa-568 anti-rabbit secondary antibody (Figure 9A). 		IHC-P Human
Kempf A, Tews B, Arzt ME et al. The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity. PLoS Biol. 2014 Jan 1 [PMID: 24453941] (WB, Mouse) WB Mouse

Review for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-26691:
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Images Ratings Applications Species Date Details
Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
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reviewed by:
Verified Customer
 IF Human 07/11/2018
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Summary

ApplicationImmunofluorescence
Sample TestedC42B prostate cell line,PC3 prostate cancer cell line
SpeciesHuman
Lot01013168A-04

Comments

Comments1:200 dilution of the antibody used

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Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
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 Verified Customer
07/11/2018
Application: IF
Species: Human
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Bioinformatics

Gene Symbol S1PR2
Uniprot