S1P2/EDG-5/S1PR2 Antibody - BSA Free

Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.

Immunocytochemistry/Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear staining. Image from verified customer review.

Increased S1PR2 accumulation in mouse TAD lesions. Representative immunohistochemistry images and the corresponding analysis of mouse S1PR2 (A,C) and S1PR1 (B,D) in TAD lesions (n = 6) and control thoracic aorta (n = 6). Representative immunoblot and the corresponding analysis of mouse S1PR2 (E,F) and S1PR1 (E,G) in TAD lesions (n = 6) and control thoracic aorta (n = 6). Scale: 200 μm, inset: 50 μm. Data are expressed as means +/- SD. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fcvm.2021.748486/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Increased Sphingosine-1-phosphate receptor 2 (S1PR2) accumulation in human thoracic aortic dissection (TAD) lesions. Representative immunohistochemistry images and the corresponding analysis of human S1PR2 (A,C) and S1PR1 (B,D) in TAD lesions (n = 6) and normal thoracic aorta (n = 3). Representative immunoblot and the corresponding analysis of human S1PR2 (E,F) and S1PR1 (E,G) in TAD lesions (n = 10) and normal thoracic aorta (n = 6). Scale: 200 μm, inset: 50 μm. Data are expressed as means +/- SD. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fcvm.2021.748486/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Po, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

S1P2/EDG-5/S1PR2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.
• Predicted Species: Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: S1PR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 25621765)
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody - BSA Free

• AGR16
• EDG5
• EDG-5
• endothelial differentiation G-protein coupled receptor 5
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
• GPCR13
• GPCR18
• H218
• LPB2
• S1P receptor 2
• S1P receptor EDG5
• S1P receptor Edg-5
• S1P2
• S1PR2
• sphingosine 1-phosphate receptor 2
• sphingosine 1-phosphate receptor Edg-5
• sphingosine-1-phosphate receptor 2

Background

This EDG5 protein is a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)(8)

Publications using NBP2-26691

• Jiang H, Huang T, Yu Y et al. Characterization of a S1PR2 specific 11C-labeled radiotracer in streptozotocin-induced diabetic murine model Nuclear Medicine and Biology 2023-07-01 [PMID: 37556928] (IHC-Fr, Mouse)
• Xue J, Lin J, Liu Z et al. Alleviating early demyelination in ischaemia/reperfusion by inhibiting sphingosine-1-phosphate receptor 2 could protect visual function from impairment Brain pathology (Zurich, Switzerland) 2023-05-04 [PMID: 37142391] (IHC, WB, Mouse)
• Janneh AH, Kassir MF, Atilgan FC et al. Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis Cell Reports 2022-12-06 [PMID: 36476873] (Western Blot, Mouse)
• Xiao P, Gu J, Xu W et al. RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction Autophagy 2022-03-09 [PMID: 35263212] (IF/IHC, Rat)
• Pan G, Liao M, Dai Y et al. Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture Frontiers in Cardiovascular Medicine 2021-12-17 [PMID: 34977175] (ICC/IF, IF/IHC, WB, Human)
• Tran HB, Maiolo S, Harper R Et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels Cell biology international 2021-08-04 [PMID: 34347342] (ICC/IF, Rat)
• Gu Y, Shea J, Slattum G et al. Defective apical extrusion signaling contributes to aggressive tumor hallmarks Elife. 2015-02-11 [PMID: 25621765] (IHC-P, Human)
  
  Details:
  S1P2/EDG-5/S1PR2 antibody used for IHC-P staining of formalin-fixed, paraffin-embedded tissue sections of Human Pancreatic Ductal Adenocarcinomas /PDACs - heat induced antigen retrieval with 10 mM sodium citrate for 20 minutes, blocking with 5% BSA/0.5% Tween-20 in PBS for 4 hours, primary incubated for ON at 4C, detection with Alexa-568 anti-rabbit secondary antibody (Figure 9A).
• Kempf A, Tews B, Arzt ME et al. The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity. PLoS Biol. 2014-01-01 [PMID: 24453941] (WB, Mouse)

Review for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Dimitra Kalamida IF Human 07/11/2018

Summary

• Application: Immunofluorescence
• Sample Tested: C42B prostate cell line,PC3 prostate cancer cell line
• Species: Human
• Lot: 01013168A-04

Comments

• Comments: 1:200 dilution of the antibody used

Bioinformatics

• Gene Symbol: S1PR2
• Uniprot:
  • O95136(Human)