S1P2/EDG-5/S1PR2 Antibody

Images

 
Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and ...read more
Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

S1P2/EDG-5/S1PR2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.
Predicted Species
Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S1PR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID 25621765)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-26691 in the following applications:

Publications
Read Publications using
NBP2-26691 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody

  • AGR16
  • EDG5
  • EDG-5
  • endothelial differentiation G-protein coupled receptor 5
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
  • GPCR13
  • GPCR18
  • H218
  • LPB2
  • S1P receptor 2
  • S1P receptor EDG5
  • S1P receptor Edg-5
  • S1P2
  • S1PR2
  • sphingosine 1-phosphate receptor 2
  • sphingosine 1-phosphate receptor Edg-5
  • sphingosine-1-phosphate receptor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
WB
(2)
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Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 5 of 5.
Publications using NBP2-26691 Applications Species
Xiao P, Gu J, Xu W et al. RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction Autophagy Mar 9 2022 [PMID: 35263212] (IHC, Rat) IHC Rat
Pan G, Liao M, Dai Y et al. Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture Frontiers in Cardiovascular Medicine Dec 17 2021 [PMID: 34977175] (IHC, WB, ICC/IF, Human) IHC, WB, ICC/IF Human
Tran HB, Maiolo S, Harper R Et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels Cell biology international Aug 4 2021 [PMID: 34347342] (ICC/IF, Rat) ICC/IF Rat
Gu Y, Shea J, Slattum G et al. Defective apical extrusion signaling contributes to aggressive tumor hallmarks Elife. 2015 Feb 11 [PMID: 25621765] (IHC-P, Human)

Details:
S1P2/EDG-5/S1PR2 antibody used for IHC-P staining of formalin-fixed, paraffin-embedded tissue sections of Human Pancreatic Ductal Adenocarcinomas /PDACs - heat induced antigen retrieval with 10 mM sodium citrate for 20 minutes, blocking with 5% BSA/0.5% Tween-20 in PBS for 4 hours, primary incubated for ON at 4C, detection with Alexa-568 anti-rabbit secondary antibody (Figure 9A).
IHC-P Human
Kempf A, Tews B, Arzt ME et al. The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity. PLoS Biol. 2014 Jan 1 [PMID: 24453941] (WB, Mouse) WB Mouse

Review for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-26691:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence S1P2/EDG-5/S1PR2 NBP2-26691
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4
reviewed by:
Verified Customer
IF Human 07/11/2018
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Summary

ApplicationImmunofluorescence
Sample TestedC42B prostate cell line,PC3 prostate cancer cell line
SpeciesHuman
Lot01013168A-04

Comments

Comments1:200 dilution of the antibody used

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)

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Diseases for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)

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Pathways for S1P2/EDG-5/S1PR2 Antibody (NBP2-26691)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
07/11/2018
Application: IF
Species: Human

Bioinformatics

Gene Symbol S1PR2
Uniprot