PAPSS1 Antibody - BSA Free

Western Blot: PAPSS1 Antibody [NBP2-97681] - Anti-PAPSS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 72 kDa

Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-97681] - Immunochemical staining of human PAPSS1 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: PAPSS1 Antibody [NBP2-97681] - PAPSS1 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate 4 ul anti-PAPSS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PAPSS1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 72 kDa

Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-97681] - Immunofluorescence staining of PAPSS1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97681 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.

Immunohistochemistry-Paraffin: PAPSS1 Antibody - BSA Free [NBP2-97681] - Immunochemical staining of human PAPSS1 in human gallbladder with NBP2-97681 at 1:500 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PAPSS1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PAPSS1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPSS1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PAPSS1 Antibody - BSA Free

• 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• ATPSK1SK 1
• bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• EC 2.7.1.25
• PAPS synthase 1
• PAPSS 1
• PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1
• SK1
• Sulfurylase kinase 1

Background

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAPSS1